Helios/UltraGRO™ GMP grade/HPCPLCGL50, HPCPLCGL10, HPCPLCGL05,Helios,,Helios,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Helios/UltraGRO™ GMP grade/HPCPLCGL50, HPCPLCGL10, HPCPLCGL05

产品编号：HPCPLCGL50,HPCPLCGL10,HPCPLCGL05

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Helios

公司：Helios

公司分类：

商品介绍

Overview
Application
Safety Information

Description:

UltraGRO™ cell culture supplement is a non-xenogeneic, animal serum-free, and heparin-requiring media supplement for replacing FBS (fetal bovine serum) to support cell expansion from research through clinical trials to commercial use. UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs).

Specification:

1. Appearance: Yellow to Buff liquid2. Total Protein: 4.0 - 8.0 g/dL3. pH: 6.5 - 8.54. Osmolality: 275 - 355 mOsm/Kg5. Endotoxin: ≤ 10 EU/mL6. Mycoplasma: Not Detected7. Sterility: No Growth8. Performance Test: Test and record

Storage and Shipping Information:

UltraGRO™ is most stable when stored frozen until needed. The recommended storage temperature is -20°C or -80°C. Thaw frozen UltraGRO™ product in 37°C water bath before use. Once UltraGRO™ is thawed, it is recommended to fully use for completed medium preparation (e.g. 5%) the same day, or to divide it into single-use aliquots and store unused aliquots at -20°C or -80°C.

Packaging Information:

500mL, 100mL, 50mL

Media Form:

Liquid

Application:

For human ex-vivo tissue and cell culture processing applications.

Instructions for Use:

1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.2. We recommend seeding MSCs at approximately 3x10³~6x10³ per cm².3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.

Cell Lines:

Bone marrow mesenchymal stem cells, Adipose tissue derived mesenchymal stem cells, Umbilical cord derived mesenchymal stem cells, Other mesenchymal stem cells.

Important Information:

1. Clotting or insoluble particles may form in thawed UltraGRO™ cell culture supplement. Published research has show that particles will not alter the performance of the product.2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.

Safety Packaging information:

1. Follow the handling instructions outlined in the Material Safety Date Sheets (MSDSs). Wear appropriate protective eyewear, clothing, and gloves.2. UltraGRO™ is a cell culture supplement derived from human platelets collected from healthy donors at FDA or HC licensed blood centers. Each donor has been tested using FDA/HC-licensed tests and found nonreactive for HBsAg, Hepatitis B core antibody (anti-HBc), HIV antibody (anti-HIV-1/2), Hepatitis C antibody (anti-HCV), HTLV-1/2 antibody (anti-HTLV-1/2), Trypanosoma cruzi antibody (anti-T. cruzi), HIV-1, HCV, HBV, WNV nucleic acid testing and Syphilis microhemagglutination test. Each donation is tested for ZIKV under either and FDA licensed test or an investigational ID-NAT test and sound non-reactive.3. This product is manufactured, tested and released in compliance with the relevant GMP guidelines. This product is for in vitro use only.

品牌介绍